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Collagenous colitis in a section of large intestine, Masson’s Trichrome stain using aniline blue as the counter(/collagen) stain.
I’m trying a thing where I post about a laboratory test every week (how it’s done, what it means, why we do it, etc). This week, I literally just grabbed a req, closed my eyes, and pointed to a random test. And that leads us to HemoglobinA1c. But you’re free to ask about a test in the future.
Diabetic nephropathy animation. Some time ago 3FX animation did a series of videos on diabetes. Check out their channel for more: http://www.youtube.com/user/3FXanimation
I figure at one point or another, I should stop posting so much about stool. (Wait until I post about a 72 hour fecal fat. Then you’ll be really jealous of my job haha, ick). So, here is a simple emergency room case study on a somewhat-elderly male. I probably won’t do very many of these at all, though.
Background: Immigrant, moved here almost 2 decades ago, living with a wife, daughter, and son-in-law with good family relationships and no major stresses. No suicidal ideations, but had a history of soap ingestion and a distant history of alcohol abuse.
He was brought to emergency by his son after he drank some Fleecy and Rug Doctor mixed in with some atarax and restoril (anti-anxiety medication) which apparently tasted like grapefruit juice. BP was 210/110, he was obtunded and close to a state of unconsciousness.
SEM of bacteria in deer stool.
I picked it for the campylobacter-like organisms in there (brown corkscrews). Despite being fastidious and sometimes tricky to grow, a good lab should be able to isolate more Campylobacter sp. than shigella and salmonella combined since it is the most common bacterial cause of diarrhea in humans.
One of the worst things about being a lab tech is how easy it is to forget that the tube you are holding is a patient, especially when you have the liberty of working in a larger hospital where you don’t do your own collections (instead we have lovely lab assistants). We are trained for collections just in case we end up working in a rural hospital, and all of my best and worst stories come from collections training because it put it face to the sample and I remember them.
Coloured SEM of a macrophage engulfing a mauve Leishmania sp. parasite.
Epidermophyton floccosum, wet prep.
The person who was teaching me mycology had an incredible accent and for the longest time I thought she was telling me to look for “puddle shaped macroconidia”. I couldn’t figure out for the life of me why anyone would describe anything as puddle shaped. It wasn’t until the end that I realized she was saying paddle shaped. Oops. They remain “puddles” in my mind though.
Epidermophyton floccosum is one of the dermatophytes, fungi that cause superficial infections of hair, skin, and nails. They generally aren’t a big deal, but there’s a lot of money in antifungals because man, do people hate dandruff and athlete’s foot.
Hyaline cartilage, H&E stain.
Though the photo’s not part of the trachea, the first time I saw this was in a section of trachea. I immediately had one of those trainwreck moments. “Euuuugh, it’s like thousands of eyes staring back at me this is so gross thisistheworstpartofhistoever!” but never managed to look away. I got over it quickly enough. There are worse things in histo, like 4AM shifts (I can do mornings, just not that kind of morning). Plus you don’t even really use your microanatomy in the end.