The Kleihauer Betke Test:

A peripheral blood smear of the mom’s blood is prepared and fixed in 80% ethanol. It is then treated with acid (McIlvaine’s buffer) and stained with eosin and hematoxylin. Fetal cells will remain in tact and stain bright pink because of the high HbF/fetal hemoglobin content which resists acid elution. Adult hemoglobin in maternal cells will elute out, leaving faintly staining “ghost cells”. The percental of fetal cells per 2000 cells is used to determine how much extra WinRho (in 300mg vials) is required:

Vials = %fetal cells x maternal blood volume (~5000mL)/30mL

Rounded to the nearest whole number + 1 extra. But that one extra is usually the shot they automatically gave her after she gave birth.

1. satoshi-is-mine reblogged this from fyeahmedlab
2. shoodii reblogged this from medical-lab-minds and added:

   I will need this later. :)

3. excors likes this
4. potassiumhydride likes this
5. queendan reblogged this from fyeahmedlab
6. oseifofie likes this
7. rxndra likes this
8. crayonaize likes this
9. tokenblonde likes this
10. sensingsarcasm likes this
11. snowhyte reblogged this from fyeahmedlab
12. anatomicalsense reblogged this from fyeahmedlab
13. idrewyouasquirrelbecauseiloveyou reblogged this from fyeahmedlab
14. satyreetbacchante reblogged this from fyeahmedlab
15. lafermeassurance likes this
16. satyreetbacchante likes this
17. cindyzilla likes this
18. haerins likes this
19. red-roman likes this
20. fyeahmedlab reblogged this from medical-lab-minds
21. pseudobot likes this
22. fuckyeaawkwardness reblogged this from biognosis
23. fuckyeaawkwardness likes this
24. biognosis reblogged this from scientificthought
25. where-there-is-music likes this
26. jeezypete reblogged this from scientificthought
27. jennypear likes this
28. scientificthought reblogged this from medical-lab-minds
29. lifeispandemic reblogged this from medical-lab-minds
30. biocas likes this
31. sciencenote likes this
32. medical-lab-minds reblogged this from statlab
33. the-ordinary-nerd likes this
34. drowninginthenile likes this
35. rotiferola likes this
36. spongetrick8 likes this
37. statlab posted this